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Plague
(Stranger) 05-24-03 08:20 No 435109 |
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Spectrophotofluorometer of any use? | Bookmark | |||||
Simple enough question, research has proven interesting, but would like to hear from those who know better... Would a spectrophotofluorometer be of any use for analysis? |
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blaaky (Hive Bee) 05-24-03 21:44 No 435223 |
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i don't know of how much use... | Bookmark | |||||
All the spectrophotometer can tell you is the concentration of a colored species in liquid... I mean we did some quantitative analysis with it in a chem lab. Some ion forms a colored complex, then do dilutions and a few simple calculations and you have the molarity of the ion. *shrug* -blaaky If you want divine justice, die. |
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SpicyBrown (Hive Bee) 05-25-03 11:35 No 435364 |
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Actually, if I understand ... | Bookmark | |||||
Actually, if I understand "spectrophotofluorometer" correctly.. The device in question is a fluorescence soectrometer, which does not tell you the concentration of a colored species in solution. A fluorescence spectrophotometer measures emission, not absorption/transmittance. Such a thing probably is not of a whole lot of use for like, determining the identity of an unknown substance, unless the substance you're looking for fluoresces, and you know what its emission spectrum looks like.. -SpicyBrown |
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