Xenonpill (Stranger)
02-11-01 20:00
No 172891
      Enzyme Sequences  Bookmark   

These are from The Resonance Project v2.15 winter 97/98.
Some of the article is available on the resonance project's website.

Tryptophan Hydroxylase:
Amino Acids: F,S,Q,E,I,G,L,A
Nucleuotides (human, rat, rabbit, mouse): TTC,TCC,CAA,GAA,ATT,GGC,CTG,GCT

Tryptophan Decarboxylase:
Amino Acids: G,K,E,M,V,D,Y
Nucleotides:
Human: GGG,AAG,GAG,ATG,GTG,GAT,TAC
Cow:   GGG,AAG,GAG,ATG,GTG,GAT,TAC
Rat:   GGG,AAG,GAG,ATG,GTG,GAT,TAT

Hydroxyindole O-Methyltransferase:
Amino Acids: D,A,V,R,E,G,R,N
Nucleotides:
Human:    GAC,GCC,GTG,AGA,GAA,GGA,AGG,AAC
Chicken:  GAT,GCT,GTG,AGA,GAA,GGA,AGA,AAT

Unfortunatly, no references were supplied in the print article or at www.resproject.com, however, it may be possible to get the refs by bugging someone at the resonance project.
KrZ, would this kind of thing help you make a yeast that produces gobs of DMT when mixed with water?

Xenonpill
 
 
 
 
    KrZ
(Hallucinogenius)
02-12-01 07:59
No 172988
      Re: Enzyme Sequences  Bookmark   

Tryptophan->Tryptamine
DEFINITION  C.roseus tdc gene for tryptophan decarboxylase.
ACCESSION   X67662
/translation="MGSIDSTNVAMSNSPVGEFKPLEAEEFRKQAHRMVDFIADYYKN                   VETYPVLSEVEPGYLRKRIPETAPYLPEPLDDIMKDIQKDIIPGMTNWMSPNFYAFFP                    ATVSSAAFLGEMLSTALNSVGFTWVSSPAATELEMIVMDWLAQILKLPKSFMFSGTGG                  GVIQNTTSESILCTIIAARERALEKLGPDSIGKLVCYGSDQTHTMFPKTCKLAGIYPN                    NIRLIPTTVETDFGISPQVLRKMVEDDVAAGYVPLFLCATLGTTSTTATDPVDSLSEI               ANEFGIWIHVDAAYAGSACICPEFRHYLDGIERVDSLSLSPHKWLLAYLDCTCLWVKQ             PHLLLRALTTNPEYLKNKQSDLDKVVDFKNWQIATGRKFRSLKLWLILRSYGVVNLQS                    HIRSDVAMGKMFEEWVRSDSRFEIVVPRNFSLVCFRLKPDVSSLHVEEVNKKLLDMLN                     STGRVYMTHTIVGGIYMLRLAVGSSLTEEHHVRRVWDLIQKLTDDLLKEA"


DEFINITION  Oryctolagus cuniculus indolethylamine N-methyltransferase (INMT) mRNA, complete cds.
ACCESSION   AF077826
/translation="MEGGFTGGDEYQKHFLPRDYLNTYYSFQSGPSPEAEMLKFNLEC                  LHKTFGPGGLQGDTLIDIGSGPTIYQVLAACESFKDITLSDFTDRNREELAKWLKKEP                     GAYDWTPALKFACELEGNSGRWQEKAEKLRATVKRVLKCDANLSNPLTPVVLPPADCV                    LTLLAMECACCSLDAYRAALRNLASLLKPGGHLVTTVTLQLSSYMVGEREFSCVALEK                 EEVEQAVLDAGFDIEQLLYSPQSYSASTAPNRGVCFLVARKKPGS"

This can also catalyze the second methylation according to quicksilver (who has been alot of help, and I am very appreciative of).

Complete mRNAs for every step, nothing too gigantic either...
 
 
 
 
    Quicksilver
(Newbee)
02-12-01 23:22
No 173044
      Re: PSILOCIN Enzyme Sequences  Bookmark   

KrZ-
Go ahead and post my last PM if you still have it.  Maybe there are enough bees with molec' bio' background to find that last step that I couldn't.

Basically my work ended in an e.coli that produces DMT  (not psilocin) because there is no cloned enzyme that will hydroxylate the 4 position on DMT. (and thus convert DMT->psilocin) Not a total loss, but not what we were looking for.

Xenopill-
If you know of a indole-hydroxylase, or tryptamine hydroxylase that will work, you'll make me a happy bee.

KrZ, thanks for the acknowledgement.  In doing so, it reaffirms what most of us know about your strength of character already.

-quicksilver-
 
 
 
 
    Rhodium
(Chief Bee)
02-13-01 05:40
No 173087
      Re: PSILOCIN Enzyme Sequences  Bookmark   

Quicksilver: Are yuo telling me that there already exist a bacterium which can convert tryptophan to DMT? How many millimoles can a culture churn out per hour?


http://rhodium.lycaeum.org
 
 
 
 
    KrZ
(Hallucinogenius)
02-13-01 11:10
No 173127
      Re: PSILOCIN Enzyme Sequences  Bookmark   

KrZ-
        I wasn't able to get any of the articles, but I'm not sure of their worth anyway.  (since they all pre-date molecular bio/DNA techniques)

        Ironically, the textbook Biochemistry by Mathews/vanHolde Chapter 21 page 740, review question 4, actually asks for a biosynthesis of
        psilocybin from tryptophan.  Too bad there's no answers in the book.....

        Regardless, you stated:
        Tryptophan -1-> Tryptamine -2-> N-Methyl-tryptamine -3-> N,N-dimethyltryptamine -4-> psilocin -5-> psilocybin

        The enzymes for the first 3 steps are known, and the protein+DNA sequences are known too.
        1-Tryptophan->Tryptamine  
           Enzyme=aromatic amino acid decarboxylase
           AKA: AAAD,  AKA: dopadecarboxylase
           Genbank Accession# M88700 (protein sequence=AAA20894)
           Reference:Human dopa decarboxylase: localization to human chromosome 7p11 and characterization of hepatic cDNAs. Genomics 13
        (2), 469-471 (1992)

        2-Tryptamine -> N-Methyl-tryptamine
           Enzyme=Indolethylamine-N-Methyltransferase
           AKA INMT
           Genebank Accession# NM_006774
           Reference:Human indolethylamine N-methyltransferase: cDNA cloning and expression, gene cloning, and chromosomal localization.
        Genomics 61 (3), 285-297 (1999)

        3-N-Methyl-tryptamine -> N,N-DiMethyl-tryptamine
           Same as #2

        4-N,N-dimethyltryptamine -> psilocin
           There most likely isn't a human gene for this.  (otherwise we'd all be alot happier people)
           I find no references in medline for: indole-hydroxylase, trptamine-hydoxylase, or indolamine-hydroxylase, leading me to believe
        there is no human (or other species) homolog.  Dead end?  My first thoughts are maybe the psilocin step is impossible at this time, or
        at least until someone clones the gene responsible.  So where from here?  Perhaps the 5-hydroxyderivative would be more attainable.
        See 5-OH-N,N-DMT http://www.erowid.org/library/books_online/tihkal/tihkal19.shtml
        Shulgin is less than enthusiastic about this compound.
        This could be accomplished with tryptophan hydroxylase.  Worth the effort??  I'm skeptical.

        5-psilocin -> psilocybin
           Haven't looked yet.


        That's all my ramblings.  Any feedback would be much appreciated.
        Cheers.
        -quicksilver-


        In reply to:

        1. Agurell, S., Blomkvist,S. and Catalfomo, P.. "Biosynthesis of Psilocybin in Submerged Culture of Psilocybin cubensis: Part I.
        Incorporation of labeled tryptophan and tryptamine," Acta Pharm. Suecica, Vol. 3 (1966), 37-44.

        2. Agurell, S., Lars, J. and and Nilsson, C.. "Biosynthesis of Psilocybin: Part II. Incorporation of Labelled Tryptamine Derivatives, Acta
        Chemica Scandinavica, Vol. 22, No. 4 (1968), 1210-1218.

        6. Catalfomo, P. and Tyler, V. E., Jr.. "The Production of Psilocybin in Submerged Culture by Psilocybe cubensis, Lloydia, Vol. 27, No.l
        (March 11. Leung, A.Y. and Paul, A.G.. "The Relationship of Carbon and Nitrogen Nutrition of Psilocybe baecocystis to the Production of
        Psilocybin and its Analogs," Lloydia, Vol. 32, No. 1(March, 1969), 66-71.1964), 53-63.
 
 
 
 
    Lilienthal
(Moderator)
02-13-01 11:57
No 173135
      Re: PSILOCIN Enzyme Sequences  Bookmark   

Here's an interesting link for you: http://www.fortunecity.com/meltingpot/gregory/1042/. It contains a huge list of literature!
 
 
 
 
    KrZ
(Hallucinogenius)
02-13-01 14:18
No 173166
      Re: PSILOCIN Enzyme Sequences  Bookmark   

The INMTs are heavily inhibited by DMT, I'm not so sure they are going to be ideal for process fermentation.  Still you could isolate the eukaryotic mRNAs, use RT conversion to make the DNA sequence (no introns), PCR, ecUP-Plasmid insert it, et voila.  However, they don't use recombinant e. coli to make aspergillus antibiotics, and there is alot of information about mycofermentation and strain optimization for fungii...  Need to grab the Agurell articles...
 
 
 
 
    Quicksilver
(Newbee)
02-13-01 18:10
No 173203
      Re: PSILOCIN Enzyme Sequences  Bookmark   


Rhodium-

Quicksilver: Are yuo telling me that there already exist a bacterium which can convert tryptophan to DMT? How many millimoles can a culture churn out per hour?


No, but hypothetically one could 'easily' make one.  I was/am interested in a bacteria that could make psilocin, but I don't think there is a cloned cDNA for a 4'-indole-hydroxylase.   I've found a cytochome p450 (CYP2D6) that will 4'-hydroxylate debrisoquine. (but debrisoquine is not quite an indole, mind you it is similar)  And of course there are enzymes that  5'-hydroxylate indoles (such as tryptophan).  S. Agurell does seem to be one of the most knowledgable/early forefathers in the field of psilocin biosynthesis.

-quicksilver-
 
 
 
 
    Quicksilver
(Newbee)
02-13-01 18:16
No 173206
      Re: PSILOCIN Enzyme Sequences  Bookmark   

Lilienthal-
How did you find this link?
Could you post the contents of that word file please.
Thanks,
-quicksilver-
 
 
 
 
    Lilienthal
(Moderator)
02-14-01 03:02
No 173259
      Re: PSILOCIN Enzyme Sequences  Bookmark   

I searched Altavista for '+psilocybin +cloning'. The wordfile seems to be much too big to be posted here.
 
 
 
 
    bioboy
(Stranger)
02-14-01 03:36
No 173262
      Re: PSILOCIN Enzyme Sequences  Bookmark   

        1: Plant Mol Biol 1997 Aug;34(6):935-48
                                                  

                       Molecular cloning and characterization of desacetoxyvindoline-4-hydroxylase, a
                       2-oxoglutarate dependent-dioxygenase involved in the biosynthesis of vindoline in
                       Catharanthus roseus (L.) G. Don.

A 2-oxoglutarate-dependent dioxygenase (EC 1.14.11.11) which catalyzes the 4-hydroxylation of
desacetoxyvindoline was purified to homogeneity. Three oligopeptides isolated from a tryptic digest of the
purified protein were microsequenced and one oligopeptide showed significant homology to hyoscyamine 6
beta-hydroxylase from Hyoscyamus niger. A 36-mer degenerate oligonucleotide based on this peptide sequence was used to screen a Catharanthus roseus cDNA library and three clones, cD4H-1 to -3, were isolated. Although none of the three clones were full-length, the open reading frame on each clone encoded a putative protein containing the sequence of all three peptides. Primer extension analysis suggested that cD4H-3, the longest cDNA clone, was missing 156 bp at the 5' end of the clone and sequencing of the genomic clone,
gD4H-8, confirmed these results. Southern blot analysis suggested that d4h is present as a single-copy gene in C. roseus which is a diploid plant, and the significant differences in the sequence of the 3'-UTR between
cD4H-1 and -3 suggest that they represent dimorphic alleles of the same hydroxylase. The identity of the clone
was further confirmed when extracts of transformed Escherichia coli expressed D4H enzyme activity. The D4H
clone encoded a putative protein of 401 amino acids with a calculated molecular mass of 45.5 kDa and the
amino acid sequence showed a high degree of similarity with those of a growing family of
2-oxoglutarate-dependent dioxygenases of plant and fungal origin.
 
 
 
 
    KrZ
(Hallucinogenius)
02-14-01 08:18
No 173292
      Re: PSILOCIN Enzyme Sequences  Bookmark   

I pulled out some of the best parts for you quicksilver, neat stuff;

Psilocybin biosynthesis
4-Hydroxylated or 4-methoxylated indoles are very rare in nature. The only known examples beside the psilocybin-type alkaloids are the 4-hydroxylation of indole acetic acid by Aspergillus niger strains (54), methoxylated b-carbolines from Banisteriopsis argentea and Picrasma javanica, the reserpine analog venenatine from Alstonia venenata, the yohimbine analog mitragynine from Mitragyna speciosa (79, 82 p. 703), and the aminopyrimidyl-indolic meridianines from the tunicate Aplidium meridianum (25). Psilocybin has a simple structure in contrast to the former alkaloids. It is formally derived from tryptophan in five distinct biosynthetic reactions, i.e. decarboxylation, indole-hydroxylation, two N-methylations, and O-phosphorylation (Table 1). Feeding experiments with putative intermediates, analogs of them, or radioactive precursors supported the view that tryptophan decarboxylation is the first biosynthetic step and that O-phosphorylation is the final step. The sequence of the remaining intermediate reaction steps is still unclear. Some authors even suppose a biosynthetic grid with multiple routes to psilocybin (Figure 1) (13, 5, 78).

Abbreviation Full name
Decarboxylase Tryptophan Decarboxylase
Hydroxylase Tryptamine 4-Monooxygenase,
Tryptamine : Oxygen Oxidoreductase, 4-hydroxylating
Methylase Tryptamine-w-amino-methyltransferase
Phosphorylase Psilocin-O-phosphotransferase

Table 1. The psilocybin synthesizing enzymes. The full name follows standard enzyme nomenclature while the abbreviated name is used throughout this text. The enzymes may actually consist of several related enzyme with slightly different substrate specifities.

Expression cloning procedure
For a definitive evaluation of psilocybin biosynthesis, the participating enzymes need to be isolated and tested for their substrate specifities and activities. The feasible way to do this was by genetically cloning and heterologous expression.
The cloning methodology chosen is an alternative to PCR based procedures and has been devoloped and used succesfully to clone a broad range of fungal enzymes (21, 20, 15, 14, 53). It consists of ligating a cDNA library into an expression vector (pYes2), transforming a host organism (S. cerevisiae), and a screening the resulting colonies for enzyme activity (Figure 2).
The success of such expression cloning procedures depends on reliable and sensitive enzyme assays for the colony screening step. Indeed it was possible to find such assays for all enzymes of the psilocybin biosynthesis pathway (Table 2).
A second important requirement is the use of biomaterial containing high amounts of the respective enzymes mRNA. Usually this is a growing tissue containing the metabolites of interest. In this study still developing Psilocybe tampanensis sclerotia were used for mRNA extraction. They grew rapidly and reliably on a special medium and contained high amounts of psilocybin and psilocin.



Total RNA isolation from enzyme producing biomaterial
\/
mRNA preparation
\/
cDNA synthesis
\/
Ligation into E. coli / S. cerevisiae shuttle expression vector (pYes2)
\/
Amplification in E. coli
\/
Colony pooling and plasmid preparation (20 pools of 5,000 colonies each)
\/
Yeast transformations
\/\/\/\/
Enzyme activity screenings of colonies by color reactions
after inducing expression (galactose)
\/
Rescreening positive clones
\/
Cross-transformation of E. coli, insert sequencing and analysis


Figure 2. Expression cloning flow scheme. As used by Dalbøge et al. to clone a broad range of fungal enzymes.

Enzyme Screening procedure
Decarboxylase 5-fluoro-tryptophan resistance
Hydroxylase feeding tryptamines, Keller's reaction
Methylase feeding tryptamines, derivatization and removal of substrate, radioactive detection
Phosphorylase feeding psilocin, derivatization of substrate, Keller's reaction after phosphatase treatment

Table 2. Enzyme activity screening procedures. All tests can be performed on colonies to allow a parallel screening procedures. The Keller's reaction is a very sensitive and specific color reaction for hydroxylated indoles.

Mushroom media and culturing
Media ingredients and sources: dried unrefined sugar-cane extract (Rapadura, organic food or third world stores), sugar-beet syrup (75% dry matter, food stores), mixed pollen (organic or health food stores), dry yeast extract (Oxoid, England), peptone (Difco Laboratories), agar (Merck, Germany), commercial malt extract / yeast solutions "Salvator" (containing 18.3% stammwuerze) and "Hefe Weissbier dunkel" (dark unfiltered wheat beer, containing 12.4% stammwuerze, both from Paulaner, Munich, Germany).
The mycelia were cultured on Parafilm sealed MEY plates (6% malt extract syrup, 0.6% yeast extract, 1.5% agar) at 28°C ± 2°C in an incubator (6). For propagation 1 cm x 1 cm blocks were cut out and transferred to the middle of a new agar plate under sterile conditions.
Plant hormones were added as ethanolic solutions. Media containing KH2PO4, pollen, acetic acid, citric acid, and ascorbic acid medium were autoclaved after adjusting the pH. Media from beer (pH around 5.5) were autoclaved for 40 min.

Mushroom extraction
For a simple but efficient extraction of psilocin as well as psilocybin the following method was applied (98, 58, 45). 7.5 mg lyophilized mushroom material was homogenized in a 1 ml glass mortar with 250 µl methanolic extraction solvent (75% MeOH, 0.1% ascorbic acid). This suspension and 2 x 125 µl methanolic rinsings were pooled in a microcentrifuge tube. After agitation for 10 min at RT the tube was centrifuged for 2 min at 14,000 rpm at RT (5415C centrifuge), the supernatant was transferred to a fresh tube, and the pellet was resuspended in 250 µl ethanolic extraction solvent (75% EtOH, 0.1% ascorbic acid). After agitation for 10 min at RT and centrifugation as above the supernatants were pooled and stored at -20°C in an airtight microcentrifuge tube.



Reagents and procedures from the cDNA Synthesis System Kit (Gibco BRL) were used in combination with a vector primed cDNA synthesis literature method (85). This approach was not succesful.




Here's a cool transformant screening procedure;
5-Fluoro-tryptophan decarboxylase screening
For tryptophan decarboxylase screening transformed FY 73 cells were plated directly onto SC-gal containing 0.5 - 1.5 mM 5-fluoro-tryptophan and were incubated up to 6 days at 30°C.

No-Go for submerged culture;
The tested Psilocybe strains showed variable preferences for the tested media. In general, the defined Leatham’s media, sugar-cane medium, and beer-based media were poor substrates compared to malt extract based media. The submerged culture produced the highest amounts of biomass, followed by the surface cultures on soft agar (0.2%). But under both conditions the mycelia did not produce measurable amounts of alkaloids. The same was true for Ps. tampanensis submerged cultures in 6% malt extract with no supplement, 0.3% yeast extract, 2% pollen, or both added (data not shown).

Ps. cubensis, Ps. tampanensis, Ps. azurescens, and Ps. cyanescens were grown in different media. After 14 days the mycelium was harvested, weighed, extracted, standardized, and analyzed for psilocin and psilocybin content.
Ps. cubensis produced psilocin, but no psilocybin under the conditions analyzed. In contrast Ps. tampanensis and Ps. azurescens produced both alkaloids. Ps. azurescens and Ps. cyanescens were growing very slowly on all media tested.

Psilocin and psilocybin Rf values
Psilocin and psilocybin references (in slightly acidic solution, isolated from Ps. cyanescens fruiting bodies) were TL-chromatographed using various solvent systems. The plates were stained with Van Urk’s reagent (DMCA modification) and Rf values were observed (Table 8).

Solvent system Psilocin Rf Psilocybin Rf
H2O: MeOH: AcOH (50 + 50 + 1) 0.92 0.88
MeOH 0.54 0.11
Acetone 0.30 0.06
MeOH: AcOH (1 + 1) 0.33 0.13
H2O 0.88 0.47
H2O: AcOH (1 + 1) 0.88 0.76
nPrOH: H2O: AcOH (10 + 3 + 3) 0.71 0.39
CH2Cl2 0.00 0.00


Best Host;
Yeast as host organism
An important factor for a succesful expression cloning procedure is the choice of an adequate host organism. Yeasts, especially S. cerevisiae, are easy to culture and grow rapidly. As well efficient transformation techniques in common with bacteria have been developed. Otherwise they have many compartments and posttranslational processing and transport systems in common with complex multicellular eukaryotes, especially with other fungi like mushrooms. For the crosstransformation from positive clones into E. coli simple methods exist (90, 84).

REFERENCES
1. Brassinosteroids. G. Adam, V. Marquardt. Phytochem. 25: 1787 (1986)
2. Biosynthesis of psilocybin in submerged culture of Psilocybe cubensis. S. Agurell, S. Blomkvist, P. Catalfomo. Acta Pharm. Suecica 3: 37 (1966)
3. Biosynthetic studies on ergot alkaloids and related indoles. S. Agurell. Acta Pharm. Suecica 3: 71 (1966)
4. A biosynthetic sequence from tryptophan to psilocybin. S. Agurell, J. G. L. Nilsson. Tet. Lett. 9: 1063 (1968)
5. Biosynthesis of psilocybin. S. Agurell, J. G. L. Nilsson. Acta Chem. Scand. 22: 1210 (1968)
6. The influence of temperature of mycelial growth of Psilocybe, Paneolus, and Copelandia. R. W. Ames. Mycopath. et Mycol. Appl. 9: 268 (1958)
7. Occurrence of psilocybin and psilocin in certain Conocybe and Psilocybe species. R. G. Benedict, L. R. Brady, A. H. Smith, V. E. Tyler. Lloydia. 25: 156 (1972)
8. Galerina steglichii spec. nov., ein halluzinogener Häubling. H. Besl. Z. Mycol. 59: 215 (1993)
9. Quantitative analysis of psilocybin and psilocin in Psilocybe baeocystis (Singer and Smith) by high-performance liquid chromatography and by thin layer chromatography. M. W. Beug, J. Bigwood. J. Chrom. 207: 379 (1981)
10. Psilocybin and psilocin levels in twenty species from seven genera of wild mushrooms in the pacific northwest, USA. M. W. Beug, J. Bigwood. J. Ethnopharmacol. 5: 271 (1982)
11. Tryptophan als biogenetische Vorstufe des Psilocybins. A. Brack, A. Hofmann, F. Kalberer, H. Kobel, J. Rutschmann. Arch. Pharm. 294: 230 (1961)
12. The production of psilocybin in submerged culture by Psilocybe cubensis. P. Catalfomo, V. E. Tyler. Lloydia 27: 53 (1964)
13. Psilocin, bufotenine and serotonin: historical and biosynthetic observations. W. C. Chilton, J. Bigwood, R. E. Jensen. J. Psyched. Drugs 11: 61 (1979)
14. Expression cloning, purification and characterization of a b-1,4-galactanase from Aspergillus aculeatus. S. Christgau, S. Sandal, L. V. Kofod, H. Dalbøge. Curr. Genet. 27: 135 (1995)
15. Pectin methyl esterase from Aspergillus aculeatus: expression cloning in yeast and characterization of the recombinant enzyme. S. Christgau, L. V. Kofod, T. Halkier, L. N. Andersen, M. Hockauf, K. Dörreich, H. Dalbøge, S. Kauppinen. Biochem. J. 319: 705 (1996)
16. Determination of psilocybin in Psilocybe semilanceata using high-performance liquid chromatography on a silica column. A. L. Christiansen, K. E. Rasmussen, F. Tønnesen. J. Chrom. 210: 163 (1981)
17. Screening of hallucinogenic mushrooms with high-performance liquid chromatography and multiple detection. A. L. Christiansen, K. E. Rasmussen. J. Chrom. 270: 293 (1983)
18. Ultra-fast alkaline lysis plasmid extraction (UFX). R. S. Cormack, I. E. Somssich. Elsevier Techn. Tips Online (1997)
19. Carbon source dependent differences in the composition of the cell walls of the basidiomycete Picnoporus cinnabarinus. J. A. Cury, D. Amaral. Can. J. Microbiol. 23: 1313 (1977)
20. Using molecular techniques to identify new microbial biocatalysts. H. Dalbøge, L. Lange. Trends Biotech. 16: 265 (1998)
21. A novel method for efficient expression cloning of fungal enzyme genes. H. Dalbøge, H. P. Heldt-Hansen. Mol. Gen. Genetics 243: 253 (1994)
22. Second-strand cDNA synthesis with E. coli DNA polymerase I and RNase H: the fate of information at the mRNA 5’ terminus and the effect of E. coli DNA ligase. J. M. D’Alessio, G. F. Gerard. Nucl. Acid Res. 16: 1999 (1988)
23. Ligation of EcoRI endonuclease generated DNA fragments into linear and circular structures. A. Dugaiczyk, H. W. Boyer, H. M. Goodman. J. Mol. Biol. 96: 171 (1975)
24. A highly sensitive and non-lethal beta-galactosidase plate assay for yeast. H. M. Duttweiler. Trends Gen. 12: 340 (1996)
25. Indole alkaloids from the tunicate Aplidium meridianum. L. H. Franco, E. B. Joffe, L. Puricelli, M. Tatian, A. M. Seldes, J. A. Palerma. J. Nat. Prod. 61: 1130 (1998)
26. Der erste Nachweis des Vorkommens von Psilocybin in Rißpilzen. J. Gartz, G. Drewitz. Z. Mycol. 51: 199 (1985)
27. Zur Untersuchung von Psilocybe semilanceata (Fr.) Kumm. J. Gartz. Pharmazie 40: 506 (1985)
28. Vergleichende dünnschichtchromatographische Untersuchung zweier Psilocybe- und einer halluzinogenen Inocybeart. J. Gartz. Pharmazie. 40: 134 (1985)
29. Dünnschichtchromatographische Analyse der Inhaltsstoffe von Pilzen der Gattung Stropharia. J. Gartz. Pharmazie. 40: 134 (1985)
30. Quantitative Bestimmung der Indolderivate von Psilocybe semilanceata (Fr.) Kumm. J. Gartz. Biochem. Physiol. Pflanzen 181: 117 (1986)
31. Vorkommen von Psilocybin und Baeocystin in Fruchtkörpern von Pluteus salicinus. J. Gartz. Planta Med. 290 (1987)
32. Variation der Indolalkaloide von Psilocybe cubensis durch unterschiedliche Kulturbedingungen. J. Gartz. Beiträge zur Kenntnis der Pilze Mitteleuropas 3: 257 (1987)
33. Biotransformation of tryptamine in fruiting body of Psilocybe cubensis. J. Gartz. Planta Medica 55: 249 (1988)
34. Biotransformation of tryptamine derivatives in mycelial cultures of Psilocybe. J. Gartz. J. Basic Microbiol. 29: 347 (1989)
35. Analysis and cultivation of fruit bodies and mycelia of Psilocybe bohemica. J. Gartz, G. K. Müller. Biochem. Physiol. Pflanzen. 184: 337 (1989)
36. Analyse der Indolderivate in Fruchtkörpern und Mycelien von Paneolus subalteatus (Berk. & Br) Sacc. J. Gartz. Biochem. Physiol. Pflanzen 184: 171 (1989)
37. Bildung und Verteilung der Indolalkaloide in Fruchtkörpern, Mycelien und Sklerotien von Psilocybe cubensis. J. Gartz. Beiträge zur Kenntnis der Pilze Mitteleuropas 5: 167 (1989)
38. Growth-promoting effect of a brassinosteroid in mycelial cultures of the fungus Psilocybe cubensis. J. Gartz, G. Adam, H.-M. Vorbrodt. Naturw. 77: 388 (1990)
39. Einfluß von Phosphat auf Fruktifikation und Sekundärmetabolismen der Myzelien von Psilocybe cubensis, Psilocybe semilanceata und Gymnopilus purpuratus. J. Gartz. Z. Mycol. 57: 149 (1991)
40. Ethnomycology, biochemistry, and cultivation of Psilocybe samuiensis Guzmán, Bandala and Allen, a new psychoactive fungus from Koh Samui, Thailand. J. Gartz, J. W. Allen, M. D. Merlin. J. Ethnopharmacol. 43: 73 (1994)
41. Occurence of psilocybin and psilocin in Psilocybe pseudobullacea (Petch) Pegler from the Venezuelan Andes. V. Marcano, A. Morales Méndez, F. Castellano, F. J. Salazar, L. Martinez. J. Ethnopharmacol. 43: 157 (1994)
42. Transforming yeast with DNA. R. D. Gietz, R. H. Schiestl. Meth. Mol. Cell. Biol. 5: 255 (1995)
43. A chimaeric tryptophan decarboxylase gene as a novel selectable marker in plant cells. O. J. Goddijn, P. M. van der Duyn-Schouten, R. A. Schilperoort, J. H. Hoge. Plant Mol. Biol. 22: 907 (1993)
44. The plant growth regulator methyl jasmonate inhibits aflatoxin production by Aspergillus flavus. M. Goodrich-Tanrikulu, N. E. Mahoney, S. B. Rodriguez. Microbiol. (reading) 141: 2831 (1995)
45. Determination of psilocin and 4-hydroxyindole-3-acetic acid in plasma by HPLC-ECD and pharmacokinetic profiles of oral and intravenous psilocybin in man. F. Hasler, D. Bourquin, R. Brenneisen, T. Bär, F. X. Vollenweider. Pharm. Acta. Helv. 72: 175 (1997)
46. The occurence of psilocybin in Gymnopilus species. G. M. Hatfield, L. J. Valdes, A. H. Smith. Lloydia 41: 140 (1978)
47. Verfahren zur Herstellung und Gewinnung von Psilocybin und Psilocin. R. Heim, A. Hofmann, A. Brack, H. Kobel, R. Cailleux. DBP patent 1087321 (1959)
48. LSD, mein Sorgenkind. A. Hofmann. Klett-Cotta, Stuttgart, Germany (1979),
engl. transl.: LSD, my problem child. McGraw-Hill, New York (1980)
49. Psilocybin and Psilocin, zwei psychotrope Wirkstoffe aus mexikanischen Rauschpilzen. A. Hofmann, R. Heim, A. Brack, H. Kobel, A. Frey, H. Ott, Th. Petrzilka, F. Troxler. Helv. Chim. Acta 52: 1557 (1959)
50. Dephosphorylation psilocybin to psilocin by alkaline phosphatase. A. Horita, L. J. Weber. Proc. Soc. Exp. Biol. Med. 106: 32 (1961)
51. High efficiency transformation of Escherichia coli with plasmids. H. Inoue, H. Nojima, H. Okayama. Gene 96: 23 (1990)
52. Isolation of psilocybin from Psilocybe argentipes and ist determination in specimens of some mushrooms. Y. Koike, K. Wada, G. Kusano, S. Nozoe. J. Nat. Prod. 44: 362 (1981)
53. Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus. L. V. Kofod, S. Kauppinen, S. Christgau, L. N. Andersen, H. P. Heldt-Hansen, K. Dörreich, H. Dalbøge. J. Biol. Chem. 269: 29182 (1994)
54. Hydroxylation of indolyl-3-acetic acid by the fungus Aspergillus niger IBFM-F-12. K. A. Koshcheenko, T. G. Baklashova, A. G. Kozlavskii, M. U. Arinbasarov, G. K. Skriabin. Prikl. Biokhim. Mikrobiol. 13: 248 (1977)
55. Sprühreagentien. K. G. Krebs, D. Heusser, H. Wimmer. in: Dünnschichtchromatographie, Ed. E. Stahl, Springer Verlag Berlin, Heidelberg, New York, 2. ed., p. 813 (1967)
56. Gymnopilus purpuratus, ein psilocybinhaltiger Pilz adventiv im Bezirk Rostock. H. Kreisel, U. Lindequist. Z. Mycol. 54: 73 (1988)
57. High-performance liquid chromatographic determination of some psychotropic indole derivatives. R. Kysilka, M. Wurst. J. Chrom. 21: 435 (1989)
58. A novel extraction procedure for psilocybin and psilocin determination in mushroom samples. R. Kysilka, M. Wurst. Planta Med. 56: 327 (1990)
59. H. Laatsch, Department of Organic Chemistry, University of Göttingen, Germany. preliminary communication
60. A chemically defined medium for the fruiting of Lentinus edodes. G. F. Leatham. Mycologia 75: 905 (1983)
61. Effects of growth regulating substances on fungi. K. M. Leelavathy. Can. J. Microbiol. 15: 713 (1968)
62. Production of psilocybin in Psilocybe baeocystis saprophytic culture. A. Y. Leung, A. H. Smith, A. G. Paul. J. Pharm. Sci. 54: 1576 (1965)
63. Baeocystin, a mono-methyl analog of psilocybin from Psilocybe baeocystis saprophytic culture. A. Y. Leung, A. G. Paul. J. Pharm. Sci. 56: 146 (1967)
64. Baeocystin and norbaeocystin: new analogs of psilocybin from Psilocybe baeocystis. A. L. Leung, A. G. Paul. J. Pharm. Sci. 57: 1667 (1968)
65. The relationship of carbon and nitrogen nutrition of Psilocybe baeocystis to the production of psilocybin and ist analogs. A. Y. Leung, A. G. Paul. Lloydia 32: 66 (1969)
66. Molecular cloning. J. Sambrook, E. F. Fritsch, T. Maniatis. Cold Spring Harbour Laboratory Press (1989)
67. Dextran blue as an aid for DNA precipitation and gel loading. U. Matysiak-Scholze, S. Dimmeler, M. Nehls. Elsevier Techn. Tips Online (1996)
68. Psilocybe semilanceata (Fr.) Quel. (Spitzkegeliger Kahlkopf). H. Michaelis. Z. Pilzkunde 43: 305 (1977)
69. Multiple Molecular forms of diarylpropane oxygenase, an H2O2 requiring, lignin degrading enzyme from Phanerochaete chrysosporium. V. Renganathan, K. Miki, M. H. Gold. Arch. Biochem. Biophys. 241: 304 (1985)
70. Permeabiliation of microorganisms by Triton X-100. G. F. Miozzari, P. Niederberger, R. Hütter. Anal. Biochem. 90: 220 (1978)
71. Interrelationship of phosphate nutrition, nitrogen metabolism, and accumulation of key secondary metabolites in saprophytic cultures of Psilocybe cubensis, Psilocybe cyanescens, and Paneolus campanulatus. J. M. Neal, R. G. Benedict, L. R. Brady. J. Pharm. Sci. 57: 1661 (1968)
72. Pharmacotheon. J. Ott. Natural Products Co., Kennewick, WA (1993)
73. Transformation of E. coli using homopolymer-linked plasmid chimeras. S. L. Peacock, C. M. McIver, J. J. Monahan. Biochim. Biophys. Acta 655: 243 (1981)
74. Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis. S. Pedersen-Bjergaard, E. Sannes, K. E. Rasmussen, F. Tønnesen. J. Chrom. 694: 375 (1997)
75. Psilocybian mycetismus with special reference to Paneolus. S. H. Pollock. J. Psyched. Drugs 8: 43 (1976)
76. GLC-mass spectral analysis of psilocin and psilocybin. D. B. Repke, D. T. Leslie, D. M. Mandell, N. G. Kish. J. Pharm. Sci. 66: 743 (1977)
77. Baeocystin in Psilocybe semilanceata. D. B. Repke. J. Pharm. Sci. 66: 113 (1977)
78. Baeocystin in Psilocybe, Conocybe and Paneolus. D. B. Repke. Lloydia 40: 566 (1977)
79. Psilocin analogs. III. Synthesis of 5-methoxy- and 5-hydroxy-1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indoles. D. B. Repke, W. J. Ferguson. J. Het. Chem. 19: 845 (1982)
80. Site of action of growth inhibitory tryptophan analogues in Catharanthus roseus cell suspension cultures. F. Sasse, M. Buchholz, J. Berlin. Z. Naturforsch. 38c: 910, 916 (1983)
81. Pihkal. A. Shulgin, A. Shulgin. Transform Press, Berkeley, CA (1991)
82. Tihkal. A. Shulgin, A. Shulgin. Transform Press, Berkeley, CA (1997)
83. Psilocybin in Fruchtkörpern von Inocybe aeruginascens. M. Smerdzieva, M. Wurst, T. Koza, J. Gartz. Planta Med. 83 (1986)
84. A rapid and inexpensive method for isolation of shuttle vector DNA from yeast for the transformation of E. coli. R. Soni, J. A. H. Murray. Nucl. Acid. Res. 20: 5852 (1992)
85. A highly efficient directional cDNA cloning method utilizing an asymmetrically tailed linker-primer plasmid. N. Spickofsky, R. F. Margolskee. Nucl. Acid Res. 19: 7105 (1991)
86. The mushroom cultivator. P. Stamets, J. S. Chilton. Agarikon Press, Olympia, WA (1983)
87. Occurence of 5-hydroxylated indole derivatives in Paneolina foenescii (Fries) Kuehner from various origin. T. Stijve, C. Hischenhuber, D. Ashley. Z. Mycol. 50: 361 (1984)
88. Psilocin, psilocybin, serotonin and urea in Paneolus cyanescens from various origin. T. Stijve. Persoonia 15: 117 (1992)
89. Convenient and effective methods for in vitro cultivation of mycelium and fruiting bodies of Lentinus edodes. Y. H. Tan, D. Moore. Mycol. Res. 96: 14077 (1992)
90. A simple method for rescuing autonomous plasmids from fission yeast. A. Topal, S. Karaer, G. Temizkan. Elsevier Techn. Tips Online (97)
91. Basic yeast methods. J. H. Toyn. Meth. Mol. Cell. Biol. 5: 249 (1995)
92. Influence of plant hormones on a wood-rotting fungus, Coriolus versicolor. S. I. Tsujiyama, J. I. Azuma, K. Okamura. Transact. Mycol. Soc. Japan 34: 369
93. Occurence of serotonin in a hallucinogenic mushroom. V. E. Tyler, JR. Science. 128: 718 (1958)
94. Exogenous regulators in the mycelium of Pleurotus ostreatus after exogenous application. K. Vinklarkova, Z. Sladky. Folia Microbiol. Praha 23: 55 (1978)
95. Mushrooms, Russia and history. Volumes 1 and 2. V. P. Wasson, R. G. Wasson. Pantheon Books, New York (1957)
96. A new psilocybian species of Copelandia. R. A. Weeks. J. Nat. Prod. 42: 469 (1979)
97. Analysis of psychotropic compounds in fungi of the genus Psilocybe by reversed phase high-performance liquid chromatography. M. Wurst, M. Semerdzieva, J. Vokoun. J. Chrom. 286: 229 (1984)
98. Analysis and isolation of indole alkaloids of fungi by high-performance liquid chromatography. M. Wurst, R. Kysilka, T. Koza. J. Chrom. 593: 201 (1992)
 
 
 
 
    Quicksilver
(Newbee)
02-20-01 15:47
No 174529
      Re: PSILOCIN Enzyme Sequences  Bookmark   

Bioboy,
Thanks for the ref!  What do you think the odds of this enzyme working on a more simple compound (tryptamine) compared to the one the authors used (desacetoxyvindoline)  ??

Don't suppose anybody has molecular ligand-binding software they want to share? (Windows or MacOS)
(e.g. Sybyl, Autodock, Insight   ...but those are all unix i think??)


-quicksilver-
 
 
 
 
    stormwind
(Stranger)
03-03-01 00:06
No 176271
      Re: Enzyme Sequences  Bookmark   

Man Oh Man - when you guys get these yeasts bred and multiplied, please let J***F or someone market some - I'm kinda short on realchem practical knowledge, but I could handle some sourdough starter without blowing the house up I think...


"I held my nose I closed my eyes
I took a drink
I didn't know if it was day or night..."
 
 
 
 
    ZyGoat
(Stranger)
03-03-01 05:36
No 176312
      Re: PSILOCIN Enzyme Sequences  Bookmark   

This is probably a stupid question, but why go to all the trouble of creating a transgenic psilocin producing yeast/bacteria when there already exists a load of fungal organisms capable of this?
 
 
 
 
    stormwind
(Stranger)
03-03-01 15:17
No 176354
      Re: PSILOCIN Enzyme Sequences  Bookmark   

yeast is cheap
yeast is easy to grow
yeast works fast
A neat culture might be a big barrel held in the dark at the correct temp
theoretical end products are easily extracted
yeast isn't banned anywhere, all strains are as far as FOAF knows universally legal...
even if some were not it would take a microbiologist to tell which ones, IF he could without DNA sequencing...
And bread is always gonna be OTC, is how this non-chemist hick sees it...so might be useful to legitimate researchers for production of theoretical legal compounds...
Man I am gonna go find a home brewers site to study...
Hows this sound, "Stinging Bee Rainbow Beer, don't drink too much, or you'll bee up all night!"
 
 
 
 
    Teonanacatl
(Hive Bee)
03-24-01 13:42
No 180405
      Re: PSILOCIN Enzyme Sequences  Bookmark   

About the inhibition of INMT...best thing would be to do an enzyme assay...chances are, the DMT is acting as a competetive inhibitor...however, if by chance DMT is acting non or uncompetetively, mutations to the E may remove this aspect of control....INMT from rabbit lung has the following relative activities with varying substrate:
alpha-methyltryptamine: 131%
tryptamine: 100%
norharmane: 58%
N-methyltryptamine: 44%
serotonin: 11%
beta-phenethylamine: 5%
desipramine: 4%
nortryptyline/1,2,3,4-tetrahydroisoquinoline/phenethanolamine: 3%
tyramine/(+/-)octopamine: 1%
ethylamine: 0.5%
all others are <0.5% (histamine, DMT, dopamine, epinephrine, epinine, ethanolamine, harmane-1,2,3,4-tetrahydrocarboxylic acid, harmol, melatonin, methylamine, nicotinamide, norepinephrine, salsolinol)
Note - DMT does have very low action...also points to competetive inhibition...the problem of DMT inhibiting INMT may be dealt with in other manners too...some working in the tissue culture field have come across the problem of producing alkaloids in plant material as it tends to build up toxic levels of alkaloids the plants can't handle...one method of dealing with this is to make the cells more open to the environment or to cause them to release the final products into solution...this has been done with cell damage (which also tends to elicit 2° metabolism, but tendst to slow down later growth), application of low levels of H2O2 as well as a number of synthetic compounds I can't recall right now, I think they were PEGs (polyethyleneglycols)...
I believe the thesis lilienthal gave you is very helpful, however, cloning of the E's in the pathway was not completed...another factor which must be looked at is the regulation of each of the genes...yeast is nice for this cause it tends to express genes from higher fungi with no problem...remove the gene with the 5' region attached and look for regulatory factors...or if you're lazy, remove the gene, cut off the 5' region and attach a strong, constitutively expressed promoter (ie. from 1° metabolism)...voila, lots o' E's :)



Begin with the dissolution of superfluous matters
So that desire and consciousness are free
 
 
 
 
    Teonanacatl
(Hive Bee)
03-24-01 14:01
No 180409
      Re: Enzyme Sequences  Bookmark   

Hey KrZ...I just did a translated blast search (protein-->nucleotide)...lots o' hits, your second sequence tended to have higher homology with INMTs...some are just general aromatic amino acid decarboxylases, some hits were also for the similar Tyr/Dopa decarboxylases, which are specific to these two substrates (eg. from Papaver somniferum)...you should also consider up-regulating production of S-adenosylmethionine if you are going to express the INMT at high levels, otherwise it might become a limiting factor in the reaction...anyways, here's the top hits(1st 5 from your first sequence, 2nd 5 from your second AA sequence)...now just look for conserved sequences between the enzymes that you are most interested in and see if there are any losses of this conservation in the Tyr/Dopa decarboxylases...hope this helps:

1: P17770  AROMATIC-L-AMINO-ACID DECARBOXYLASE (DOPA DECARBOXYLASE) (TRYPTOPHAN DECARBOXYLASE) BLink, PubMed, Related Sequences, Taxonomy, LinkOut 

LOCUS       DCD_CATRO     500 aa                    PLN       01-NOV-1995
DEFINITION  AROMATIC-L-AMINO-ACID DECARBOXYLASE (DOPA DECARBOXYLASE)
            (TRYPTOPHAN DECARBOXYLASE).
ACCESSION   P17770
PID         g118306
VERSION     P17770  GI:118306
DBSOURCE    swissprot: locus DCD_CATRO, accession P17770;
            class: standard.
            created: Aug 1, 1990.
            sequence updated: Aug 1, 1990.
            annotation updated: Nov 1, 1995.
            xrefs: gi: gi: 167489, gi: gi: 167490, gi: gi: 18225, gi: gi:
            18226, gi: gi: 1247291, gi: gi: 1247292, gi: gi: 68027, gi: gi:
            100170, gi: gi: 542023
            xrefs (non-sequence databases): PFAM PF00282, PROSITE PS00392
KEYWORDS    Lyase; Decarboxylase; Pyridoxal phosphate.
SOURCE      Madagascar periwinkle.
  ORGANISM  Catharanthus roseus
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; core
            eudicots; Asteridae; euasterids I; Gentianales; Apocynaceae;
            Catharanthus.
REFERENCE   1  (residues 1 to 500)
  AUTHORS   De Luca,V., Marineau,C. and Brisson,N.
  TITLE     Molecular cloning and analysis of cDNA encoding a plant tryptophan
            decarboxylase: comparison with animal dopa decarboxylases
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86 (8), 2582-2586 (1989)
  MEDLINE   89202373
  REMARK    SEQUENCE FROM N.A.
REFERENCE   2  (residues 1 to 500)
  AUTHORS   Goddijn,O.J., Lohman,F.P., de Kam,R.J., Schilperoort,R.A. and
            Hoge,J.H.
  TITLE     Nucleotide sequence of the tryptophan decarboxylase gene of
            Catharanthus roseus and expression of tdc-gusA gene fusions in
            Nicotiana tabacum
  JOURNAL   Mol. Gen. Genet. 242 (2), 217-225 (1994)
  MEDLINE   94211212
  REMARK    SEQUENCE FROM N.A.
            STRAIN=CV. MORNING MIST; TISSUE=LEAF
COMMENT     -------------------------------------------------------------------
            This SWISS-PROT entry is copyright. It is produced through a
            collaboration between the Swiss Institute of Bioinformatics and
            the EMBL outstation - the European Bioinformatics Institute.
            The original entry is available from http://www.expasy.ch/sprot
            and http://www.ebi.ac.uk/sprot
            ------------------------------------------------------------------.
            [CATALYTIC ACTIVITY] L-TRYPTOPHAN = TRYPTAMINE + CO(2) (ALSO ACTS
            ON 5-HYDROXY-L-TRYPTOPHAN AND DIHYDROXY-L-PHENYLALANINE (DOPA)).
            [COFACTOR] PYRIDOXAL PHOSPHATE.
            [SUBUNIT] HOMODIMER.
            [SIMILARITY] BELONGS TO GROUP II DECARBOXYLASES (DDC, GAD, HDC AND
            TYRDC).
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Catharanthus roseus"
                     /db_xref="taxon:4058"
                     1..500
     Protein         1..500
                     /product="AROMATIC-L-AMINO-ACID DECARBOXYLASE"
                     /EC_number="4.1.1.28"
     Site            319
                     /site_type="binding"
                     /note="PYRIDOXAL PHOSPHATE (BY SIMILARITY)."
ORIGIN     
        1 mgsidstnva msnspvgefk pleaeefrkq ahrmvdfiad yyknvetypv lsevepgylr
       61 kripetapyl peplddimkd iqkdiipgmt nwmspnfyaf fpatvssaaf lgemlstaln
      121 svgftwvssp aatelemivm dwlaqilklp ksfmfsgtgg gviqnttses ilctiiaare
      181 raleklgpds igklvcygsd qthtmfpktc klagiypnni rlipttvetd fgispqvlrk
      241 mveddvaagy vplflcatlg ttsttatdpv dslseianef giwihvdaay agsacicpef
      301 rhyldgierv dslslsphkw llayldctcl wvkqphlllr alttnpeylk nkqsdldkvv
      361 dfknwqiatg rkfrslklwl ilrsygvvnl qshirsdvam gkmfeewvrs dsrfeivvpr
      421 nfslvcfrlk pdvsslhvee vnkklldmln stgrvymtht ivggiymlrl avgsslteeh
      481 hvrrvwdliq kltddllkea
//



1: AAB39709  tryptophan decarboxylase [Camptotheca acuminata] BLink, Related Sequences, Nucleotide, Taxonomy 

LOCUS       AAB39709      498 aa                    PLN       02-JAN-1997
DEFINITION  tryptophan decarboxylase [Camptotheca acuminata].
ACCESSION   AAB39709
PID         g1763279
VERSION     AAB39709.1  GI:1763279
DBSOURCE    locus CAU73657 accession U73657.1
KEYWORDS    .
SOURCE      Camptotheca acuminata.
  ORGANISM  Camptotheca acuminata
            Eukaryota; Viridiplantae; Embryophyta; Tracheophyta; Spermatophyta;
            Magnoliophyta; eudicotyledons; core eudicots; Asteridae; Cornales;
            Cornaceae; Nyssoideae; Camptotheca.
REFERENCE   1  (residues 1 to 498)
  AUTHORS   Lopez-Meyer,M. and Nessler,C.L.
  TITLE     Tryptophan decarboxylase is encoded by two autonomously regulated
            genes in Camptotheca acuminata which are differentially expressed
            during development and stress
  JOURNAL   Unpublished
REFERENCE   2  (residues 1 to 498)
  AUTHORS   Lopez-Meyer,M. and Nessler,C.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-1996) Biology, Texas A&M University, College
            Station, TX 77843-3258, USA
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Camptotheca acuminata"
                     /db_xref="taxon:16922"
                     /tissue_type="leaf"
     Protein         1..498
                     /function="converts tryptohan to tryptamine"
                     /product="tryptophan decarboxylase"
                     /EC_number="4.1.1.28"
     CDS             1..498
                     /gene="tdc2"
                     /coded_by="U73657.1:1576..3072"
                     /note="TDC"
ORIGIN     
        1 mgsidsnydt esagqcrple peefrkqahq mvdfiadyyk niesypvlsq vepgylqsrl
       61 petapyrpep fesilkdvhk diipgvthwl spnffayfpa tvssaafvge mlctcfnavg
      121 fnwlaspael elemvvmdwl asmlklpnsf tflgtgggvi qgttseailc tliaardral
      181 esigvdsihk lvvygsdqth styakacnla gilpcnirsi rteavanfsl spdslhreie
      241 advaagmvpl ylcatvgtts ttaidslspl advandyglw fhvdaayags acicpefrhy
      301 ldgieradsl slsphkwlls yldccclwvk rpsvlvkals tdpeylknkp sesnsvvdfk
      361 dwqvgtgrrf kalrlwfvmr sygvanlqsh irsdiqmakm feefvnsdpr feivvprvfs
      421 lvcfrlnpfs ksdpcntell nrkllewvns tgqvyithtk vggvymlrfa vgatlteehh
      481 vsaawklire gadallcs
//



1: AAB39708  tryptophan decarboxylase [Camptotheca acuminata] BLink, Related Sequences, Nucleotide, Taxonomy 

LOCUS       AAB39708      502 aa                    PLN       02-JAN-1997
DEFINITION  tryptophan decarboxylase [Camptotheca acuminata].
ACCESSION   AAB39708
PID         g1763277
VERSION     AAB39708.1  GI:1763277
DBSOURCE    locus CAU73656 accession U73656.1
KEYWORDS    .
SOURCE      Camptotheca acuminata.
  ORGANISM  Camptotheca acuminata
            Eukaryota; Viridiplantae; Embryophyta; Tracheophyta; Spermatophyta;
            Magnoliophyta; eudicotyledons; core eudicots; Asteridae; Cornales;
            Cornaceae; Nyssoideae; Camptotheca.
REFERENCE   1  (residues 1 to 502)
  AUTHORS   Lopez-Meyer,M. and Nessler,C.L.
  TITLE     Tryptophan decarboxylase is encoded by two autonomously regulated
            genes in Camptotheca acuminata which are differentially expressed
            during development and stress
  JOURNAL   Unpublished
REFERENCE   2  (residues 1 to 502)
  AUTHORS   Lopez-Meyer,M. and Nessler,C.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-1996) Biology, Texas A&M University, College
            Station, TX 77843-3258, USA
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Camptotheca acuminata"
                     /db_xref="taxon:16922"
                     /dev_stage="7-day-old seedlings"
     Protein         1..502
                     /function="converts tryptophan into tryptamine"
                     /product="tryptophan decarboxylase"
                     /EC_number="4.1.1.28"
     CDS             1..502
                     /gene="tdc1"
                     /coded_by="U73656.1:189..1697"
                     /note="TDC"
ORIGIN     
        1 mgsldsnydt espasvgqfn pldpeefrkq ahcivdfiad yykniesypv lsqvdpgyrh
       61 srlgknapyr sepfesilkd vqkdiipgmt hwmspnffah fpatvssaaf vgemlctcfn
      121 svgfnwlasp aatelemvvi dwlanmlklp ksfmfsgtgg gvlqgttsea ilctliaasp
      181 mhfeivgvkt stsfvvygsd qthstyakac klagilpcni rsipttadsn fsvsplllrr
      241 aieadkaagm vplyicatvg ttsttaidpl ssladvandy gvwfhvdaay agsacicpef
      301 rhyldgiera dslslsphkw llsyldcccl wvkspsllvk alstdpeylk nqpsesksvv
      361 dykdwqvgtg rrfkalrlwf vmrsygvanl qshirtdvqm akmfegfvks dprfeilvpr
      421 vfslvcfrln pisgsdptgt ealnrklldw vnstgrvymt htkvggiyml rfavgatlte
      481 krhvssawkl ikegadvllk ed
//



1: AAG60665  tyrosine/dopa decarboxylase [Thalictrum flavum subsp. glaucum] BLink, Related Sequences, Nucleotide, Taxonomy 

LOCUS       AF314150_1    518 aa                    PLN       31-JAN-2001
DEFINITION  tyrosine/dopa decarboxylase [Thalictrum flavum subsp. glaucum].
ACCESSION   AAG60665
PID         g12620328
VERSION     AAG60665.1  GI:12620328
DBSOURCE    locus AF314150 accession AF314150.1
KEYWORDS    .
SOURCE      Thalictrum flavum subsp. glaucum.
  ORGANISM  Thalictrum flavum subsp. glaucum
            Eukaryota; Viridiplantae; Embryophyta; Tracheophyta; Spermatophyta;
            Magnoliophyta; eudicotyledons; Ranunculales; Ranunculaceae;
            Thalictrum.
REFERENCE   1  (residues 1 to 518)
  AUTHORS   Samanani,N. and Facchini,P.J.
  TITLE     Cloning and characterization of a tyrosine/dopa decarboxylase cDNA
            from the meadow rue, Thalictrum flavum ssp. glaucum
  JOURNAL   Unpublished
REFERENCE   2  (residues 1 to 518)
  AUTHORS   Samanani,N. and Facchini,P.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2000) Department of Biological Sciences,
            University of Calgary, 2500 University Drive N.W., Calgary, Alberta
            T2N 1N4, Canada
COMMENT     Method: conceptual translation supplied by author.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Thalictrum flavum subsp. glaucum"
                     /sub_species="glaucum"
                     /db_xref="taxon:150095"
                     /note="cell suspension culture"
     Protein         1..518
                     /product="tyrosine/dopa decarboxylase"
                     /EC_number="4.1.1.25"
     CDS             1..518
                     /gene="TYDC1"
                     /coded_by="AF314150.1:116..1672"
ORIGIN     
        1 mgslhvedld niskctvenp ldpeefrrqg hmmidflady yrdiekypvr sqvepgylrk
       61 eipdsapynp esietiledv hkqiipgith wqspnyfayf pssgsvagfl gemlstgfnv
      121 vgfnwmsspa atelesivmd wlgkmlklpk sflfsgnggg vlqgttceai lctltaardr
      181 mlnkigreni cklvvygsdq thcalqkaaq iagihpnnfr avpttkandy glsasalrst
      241 iledieaglv plflcatvgt tsstavdpig plckvasdys iwvhvdaaya gsacicpefr
      301 hfidgvenad sfslnahkwf fttldccclw vkepsalika lstnpeylrn kateshqvvd
      361 ykdwqialsr rframklwlv lrsygvanlr nflrshvkma knfegfiald krfeivvprt
      421 famvcfrllp prspliiktn gyqngngvyh kdesraneln rrllesinas gsaymthsmv
      481 ggvymirfav gaslteerhv ilawkvvqeh adavlatf
//



1: P54769  TYROSINE/DOPA DECARBOXYLASE 2 [INCLUDES: DOPA DECARBOXYLASE (DDC); TYROSINE DECARBOXYLASE ] BLink, PubMed, Related Sequences, Taxonomy, LinkOut 

LOCUS       TYD2_PAPSO    531 aa                    PLN       15-JUL-1999
DEFINITION  TYROSINE/DOPA DECARBOXYLASE 2 [INCLUDES: DOPA DECARBOXYLASE (DDC);
            TYROSINE DECARBOXYLASE ].
ACCESSION   P54769
PID         g1717826
VERSION     P54769  GI:1717826
DBSOURCE    swissprot: locus TYD2_PAPSO, accession P54769;
            class: standard.
            created: Oct 1, 1996.
            sequence updated: Oct 1, 1996.
            annotation updated: Jul 15, 1999.
            xrefs: gi: gi: 607746, gi: gi: 607747
            xrefs (non-sequence databases): PFAM PF00282, PROSITE PS00392
KEYWORDS    Lyase; Decarboxylase; Pyridoxal phosphate; Multigene family.
SOURCE      opium poppy.
  ORGANISM  Papaver somniferum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons;
            Ranunculales; Papaveraceae; Papaver.
REFERENCE   1  (residues 1 to 531)
  AUTHORS   Facchini,P.J. and De Luca,V.
  TITLE     Differential and tissue-specific expression of a gene family for
            tyrosine/dopa decarboxylase in opium poppy
  JOURNAL   J. Biol. Chem. 269 (43), 26684-26690 (1994)
  MEDLINE   95014524
  REMARK    SEQUENCE FROM N.A.
            STRAIN=CV. MARIANNE
COMMENT     -------------------------------------------------------------------
            This SWISS-PROT entry is copyright. It is produced through a
            collaboration between the Swiss Institute of Bioinformatics and
            the EMBL outstation - the European Bioinformatics Institute.
            The original entry is available from http://www.expasy.ch/sprot
            and http://www.ebi.ac.uk/sprot
            ------------------------------------------------------------------.
            [FUNCTION] MARGINALLY HIGHER SUBSTRATE SPECIFICITY FOR L-DOPA OVER
            L-TYROSINE.
            [CATALYTIC ACTIVITY] L-TYROSINE = TYRAMINE + CO(2).
            [COFACTOR] PYRIDOXAL PHOSPHATE.
            [SUBUNIT] HOMODIMER (BY SIMILARITY).
            [TISSUE SPECIFICITY] PREDOMINANTLY EXPRESSED IN THE ROOTS AND
            STEMS, WHILE A LOWER LEVEL EXPRESSION IS SEEN IN THE SEPALS AND
            CARPELS OF FULLY EXPANDED FLOWERS.
            [SIMILARITY] BELONGS TO GROUP II DECARBOXYLASES (DDC, GAD, HDC AND
            TYRDC).
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Papaver somniferum"
                     /db_xref="taxon:3469"
                     1..531
     Protein         1..531
                     /product="TYROSINE/DOPA DECARBOXYLASE 2 [INCLUDES: DOPA
                     DECARBOXYLASE"
                     /EC_number="4.1.1.28"
                     /EC_number="4.1.1.25"
     Site            319
                     /site_type="binding"
                     /note="PYRIDOXAL PHOSPHATE (BY SIMILARITY)."
ORIGIN     
        1 mgslntedvl enssafgvtn pldpeefrrq ghmiidflad yyrdvekypv rsqvepgylr
       61 krlpetapyn pesietilqd vtteiipglt hwqspnyyay fpssgsvagf lgemlstgfn
      121 vvgfnwmssp aatelesvvm dwfgkmlnlp esflfsgsgg gvlqgtscea ilctltaard
      181 rklnkigreh igrlvvygsd qthcalqkaa qvaginpknf raiktfkens fglsaatlre
      241 viledieagl iplfvcptvg ttsstavdpi spicevakey emwvhvdaay agsacicpef
      301 rhfidgveea dsfslnahkw ffttldcccl wvkdpsalvk alstnpeylr nkatesrqvv
      361 dykdwqials rrfrslklwm vlrsygvtnl rnflrshvkm aktfeglicm dgrfeitvpr
      421 tfamvcfrll ppktikvydn gvhqngngvv plrdenenlv lanklnqvyl etvnatgsvy
      481 mthavvggvy mirfavgstl teerhviyaw kilqehadli lgkfseadfs s
//


1: AAC97491  indolethylamine N-methyltransferase [Oryctolagus cuniculus] BLink, PubMed, Related Sequences, Nucleotide, Taxonomy 

LOCUS       AAC97491      263 aa                    MAM       22-DEC-1998
DEFINITION  indolethylamine N-methyltransferase [Oryctolagus cuniculus].
ACCESSION   AAC97491
PID         g4039109
VERSION     AAC97491.1  GI:4039109
DBSOURCE    locus AF077826 accession AF077826.1
KEYWORDS    .
SOURCE      rabbit.
  ORGANISM  Oryctolagus cuniculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Lagomorpha; Leporidae; Oryctolagus.
REFERENCE   1  (residues 1 to 263)
  AUTHORS   Thompson,M.A. and Weinshilboum,R.M.
  TITLE     Rabbit lung indolethylamine N-methyltransferase. cDNA and gene
            cloning and characterization
  JOURNAL   J. Biol. Chem. 273 (51), 34502-34510 (1998)
  MEDLINE   99069450
REFERENCE   2  (residues 1 to 263)
  AUTHORS   Thompson,M.A. and Weinshilboum,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-1998) Pharmacology, Mayo Clinic/Mayo Foundation,
            200 1st St. SW, Rochester, MN 55905, USA
COMMENT     Method: conceptual translation supplied by author.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Oryctolagus cuniculus"
                     /db_xref="taxon:9986"
                     /tissue_type="lung"
     Protein         1..263
                     /function="catalyzes the N-methylation of tryptamine"
                     /product="indolethylamine N-methyltransferase"
     CDS             1..263
                     /gene="INMT"
                     /coded_by="AF077826.1:16..807"
ORIGIN     
        1 meggftggde yqkhflprdy lntyysfqsg pspeaemlkf nleclhktfg pgglqgdtli
       61 digsgptiyq vlaacesfkd itlsdftdrn reelakwlkk epgaydwtpa lkfacelegn
      121 sgrwqekaek lratvkrvlk cdanlsnplt pvvlppadcv ltllamecac csldayraal
      181 rnlasllkpg ghlvttvtlq lssymvgere fscvalekee veqavldagf dieqllyspq
      241 sysastapnr gvcflvarkk pgs
//



1: XP_004862  indolethylamine N-methyltransferase [Homo sapiens] BLink, Related Sequences, Nucleotide, Taxonomy, LinkOut 

LOCUS       XP_004862     263 aa                    PRI       09-FEB-2001
DEFINITION  indolethylamine N-methyltransferase [Homo sapiens].
ACCESSION   XP_004862
PID         g11420979
VERSION     XP_004862.1  GI:11420979
DBSOURCE    REFSEQ: accession XM_004862.1
KEYWORDS    .
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 263)
  AUTHORS   NCBI Annotation Project.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
COMMENT     GENOME ANNOTATION REFSEQ:  This reference sequence was derived by
            automated computational analysis of NCBI genomic sequence contig
            NT_007825 using gene prediction method: Acembly.
            Supporting evidence includes similarity to: 71 proteins, 1 mRNAs
            See details in AceView
            Method: conceptual translation supplied by author.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="7"
     Protein         1..263
                     /product="indolethylamine N-methyltransferase"
     Region          4..259
                     /region_name="NNMT/PNMT/TEMT family"
                     /db_xref="CDD:pfam01234"
                     /note="NNMT_PNMT_TEMT"
     CDS             1..263
                     /gene="INMT"
                     /db_xref="LocusID:11185"
                     /db_xref="MIM:604854"
                     /coded_by="TR00067273:1..792"
ORIGIN     
        1 mkggftggde yqkhflprdy latyysfdgs pspeaemlkf nleclhktfg pgglqgdtli
       61 digsgptiyq vlaacdsfqd itlsdftdrn reelekwlkk epgaydwtpa vkfacelegn
      121 sgrweekeek lraavkrvlk cdvhlgnpla pavlpladcv ltllamecac csldayraal
      181 cnlasllkpg ghlvttvtlr lpsymvgkre fscvalekee veqavldagf dieqllhspq
      241 sysvtnaann gvcfivarkk pgp
//



1: NP_006765  indolethylamine N-methyltransferase; thioester S-methyltransferase-like [Homo sapiens] BLink, PubMed, Related Sequences, Nucleotide, Taxonomy, OMIM, LinkOut 

LOCUS       NP_006765     263 aa                    PRI       03-FEB-2001
DEFINITION  indolethylamine N-methyltransferase; thioester
            S-methyltransferase-like [Homo sapiens].
ACCESSION   NP_006765
PID         g10092584
VERSION     NP_006765.2  GI:10092584
DBSOURCE    REFSEQ: accession NM_006774.2
KEYWORDS    .
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 263)
  AUTHORS   Thompson,M.A., Moon,E., Kim,U.J., Xu,J., Siciliano,M.J. and
            Weinshilboum,R.M.
  TITLE     Human indolethylamine N-methyltransferase: cDNA cloning and
            expression, gene cloning, and chromosomal localization
  JOURNAL   Genomics 61 (3), 285-297 (1999)
  MEDLINE   20021766
   PUBMED   10552930
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF128848.1,
            AF128847.1.
            On Sep 12, 2000 this sequence version replaced gi:5803044.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p15.3-p15.2"
     Protein         1..263
                     /product="indolethylamine N-methyltransferase"
                     /note="thioester S-methyltransferase-like"
     Region          4..259
                     /region_name="NNMT/PNMT/TEMT family"
                     /db_xref="CDD:pfam01234"
                     /note="NNMT_PNMT_TEMT"
     CDS             1..263
                     /gene="INMT"
                     /db_xref="LocusID:11185"
                     /db_xref="MIM:604854"
                     /coded_by="NM_006774.2:1..792"
ORIGIN     
        1 mkggftggde yqkhflprdy latyysfdgs pspeaemlkf nleclhktfg pgglqgdtli
       61 digsgptiyq vlaacdsfqd itlsdftdrn reelekwlkk epgaydwtpa vkfacelegn
      121 sgrweekeek lraavkrvlk cdvhlgnpla pavlpladcv ltllamecac csldayraal
      181 cnlasllkpg ghlvttvtlr lpsyvvgkre fscvalekee veqavldagf dieqllhspq
      241 sysvtnaann gvccivarkk pgp
//



1: AAF18305  indolethylamine N-methyltransferase [Homo sapiens] BLink, PubMed, Related Sequences, Nucleotide, Taxonomy, OMIM, LinkOut 

LOCUS       AF128847_1    263 aa                    PRI       16-DEC-1999
DEFINITION  indolethylamine N-methyltransferase [Homo sapiens].
ACCESSION   AAF18305
PID         g6580817
VERSION     AAF18305.1  GI:6580817
DBSOURCE    locus AF128847 accession AF128847.1
KEYWORDS    .
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 263)
  AUTHORS   Thompson,M.A., Moon,E., Kim,U.J., Xu,J., Siciliano,M.J. and
            Weinshilboum,R.M.
  TITLE     Human indolethylamine N-methyltransferase: cDNA cloning and
            expression, gene cloning, and chromosomal localization
  JOURNAL   Genomics 61 (3), 285-297 (1999)
  MEDLINE   20021766
   PUBMED   10552930
REFERENCE   2  (residues 1 to 263)
  AUTHORS   Thompson,M.A., Moon,E., Kim,U.-J., Xu,J., Siciliano,M.J. and
            Weinshilboum,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-1999) Pharmacology, Mayo Clinic, 200 1st St. SW,
            Rochester, MN 55905, USA
COMMENT     Method: conceptual translation supplied by author.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p15.2-p15.3"
     Protein         1..263
                     /product="indolethylamine N-methyltransferase"
                     /name="INMT"
     CDS             1..263
                     /gene="INMT"
                     /coded_by="AF128847.1:17..808"
ORIGIN     
        1 mkggftggde yqkhflprdy latyysfdgs pspeaemlkf nleclhktfg pgglqgdtli
       61 digsgptiyq vlaacdsfqd itlsdftdrn reelekwlkk epgaydwtpa vkfacelegn
      121 sgrweekeek lraavkrvlk cdvhlgnpla pavlpladcv ltllamecac csldayraal
      181 cnlasllkpg ghlvttvtlr lpsymvgkre fscvalekge veqavldagf dieqllhspq
      241 sysvtnaann gvccivarkk pgp
//




1: BAB28594  putative [Mus musculus] BLink, PubMed, Related Sequences, Nucleotide, Taxonomy, LinkOut 

LOCUS       BAB28594      264 aa                    ROD       08-FEB-2001
DEFINITION  putative [Mus musculus].
ACCESSION   BAB28594
PID         g12850108
VERSION     BAB28594.1  GI:12850108
DBSOURCE    locus AK013010 accession AK013010.1
KEYWORDS    .
SOURCE      house mouse.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE   1  (sites)
  AUTHORS   Carninci,P. and Hayashizaki,Y.
  TITLE     High-efficiency full-length cDNA cloning
  JOURNAL   Methods Enzymol. 303, 19-44 (1999)
REFERENCE   2  (sites)
  AUTHORS   Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K.,
            Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Normalization and subtraction of cap-trapper-selected cDNAs to
            prepare full-length cDNA libraries for rapid discovery of new genes
  JOURNAL   Genome Res. 10 (10), 1617-1630 (2000)
  MEDLINE   20499374
REFERENCE   3  (sites)
  AUTHORS   Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P.,
            Konno,H., Akiyama,J., Nishi,K., Kitsunai,T., Tashiro,H., Itoh,M.,
            Kikuchi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T.,
            Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T.,
            Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y., Izawa,M., Ohara,E.,
            Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K., Tanaka,T.,
            Matsuura,S., Okazaki,Y., Muramatsu,M., Inoue,Y. and Hayashizaki,Y.
  TITLE     RIKEN integrated sequence analysis (RISA) system--384-format
            sequencing pipeline with 384 multicapillary sequencer
  JOURNAL   Genome Res. 10 (11), 1757-1771 (2000)
  MEDLINE   20530913
REFERENCE   4  (sites)
  AUTHORS   The RIKEN Genome Exploration Research Group Phase II Team and
            FANTOM Consortium.
  TITLE     Functional annotation of a full-length mouse cDNA collection
  JOURNAL   Nature 409, 685-690 (2001)
REFERENCE   5  (residues 1 to 264)
  AUTHORS   Adachi,J., Aizawa,K., Akahira,S., Akimura,T., Aono,H., Arai,A.,
            Arakawa,T., Carninci,P., Fukuda,S., Fukunishi,Y., Furuno,M.,
            Hanagaki,T., Hara,A., Hayatsu,N., Hiramoto,K., Hiraoka,T., Hori,F.,
            Imotani,K., Ishii,Y., Itoh,M., Izawa,M., Kato,H., Kawai,J.,
            Kojima,Y., Konno,H., Kouda,M., Koya,S., Kurihara,C., Matsuyama,T.,
            Miyazaki,A., Nishi,K., Nomura,K., Numazaki,R., Ohno,M., Okazaki,Y.,
            Okido,T., Owa,C., Saito,H., Saito,R., Sakai,C., Sakai,K., Sano,H.,
            Sasaki,D., Shibata,K., Shibata,Y., Shinagawa,A., Shiraki,T.,
            Sogabe,Y., Suzuki,H., Tagami,M., Tagawa,A., Takahashi,F.,
            Tanaka,T., Tejima,Y., Toya,T., Yamamura,T., Yasunishi,A.,
            Yoshida,K., Yoshino,M., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2000) Yoshihide Hayashizaki, The Institute of
            Physical and Chemical Research (RIKEN), Laboratory for Genome
            Exploration Research Group, RIKEN Gemomic Sciences Center (GSC),
            RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama,
            Kanagawa 230-0045, Japan (E-mail:genome-res@gsc.riken.go.jp,
            URL:http://genome.gsc.riken.go.jp/, Tel:81-45-503-9222,
            Fax:81-45-503-9216)
COMMENT     Please visit our web site (http://genome.gsc.riken.go.jp/) for
            further details.
            cDNA library was prepared and sequenced in Mouse Genome
            Encyclopedia Project of Genome Exploration Research Group in Riken
            Genomic Sciences Center and Genome Science Laboratory in RIKEN.
            Division of Experimental Animal Research in Riken contributed to
            prepare mouse tissues. First strand cDNA was primed with a primer
            [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was
            prepared by using trehalose thermo-activated reverse transcriptase
            and subsequently enriched for full-length by cap-trapper. cDNA went
            through one round of normalization to Rot = 7.5 and subtraction to
            Rot = 37.5. Second strand cDNA was prepared with the primer adapter
            of sequence [5'
            GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved
            with XhoI and SstI. Cloning sites, 5' end: XhoI; 3' end: SstI.
            Host: SOLR.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Mus musculus"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /db_xref="MGD:MGI:102963"
                     /db_xref="MGD:MGI:1898920"
                     /clone="2810406G07"
                     /clone_lib="RIKEN full-length enriched mouse cDNA library"
                     /dev_stage="10, 11 days embryo"
     Protein         1..264
                     /name="putative"
     CDS             1..264
                     /coded_by="AK013010.1:45..839"
ORIGIN     
        1 megkvyigge dyekeftpkd ylttyysfhs gpvaeqeivk fslqnlyqtf stggvggdvl
       61 idigsgptiy qllsacevfr eiivtdytpq nmqelqkwlk kepgaydwss ivqhaceleg
      121 drsrwqekea klrrtvtrvl rcdvtktppl gsaqvpladc vltflameca cpdidtyraa
      181 lrrlagllkp gghlvtlvtl rfqhymvgpk kfsgvyleke vvekaiqdag cqvlkcncvs
      241 lsyseaycsh dglcfvvark gpsa
//





Begin with the dissolution of superfluous matters
So that desire and consciousness are free
 
 
 
 
    Quicksilver
(Newbee)
03-24-01 19:47
No 180456
      Re: Enzyme Sequences  Bookmark   

What is missing is still the enzyme for this step:  
   
      N,N-dimethyltryptamine -> psilocin

Try a blast/medline search for that reaction and see what you get.
We already have candidates for the others. (you just dug up similar ones from different species)



you should also consider up-regulating production of S-adenosylmethionine if you are going to express the INMT at high levels, otherwise it might become a limiting factor in the reaction


Isn't this called SAMe?  It can be purchased OTC and added as needed, instead of doing some sort of biological manipulation.
-quicksilver-
 
 
 
 
    Teonanacatl
(Hive Bee)
04-10-01 12:55
No 183339
      Re: Enzyme Sequences  Bookmark   

Well...I looked...it ain't there...this isn't surprising, as the thesis on molecular biology of Psilocybe species (which is recent) noted that this step had not been characterized...there are a number of tyrosine monooxygenases and there is a hit for tryptophan-5-monooxygenase...probably the best way to get the sequence for the hydroxylation of DMT (or NMT/T; it's unknown which step happens first in-vivo) would be PCR amplification of a conserved sequence for aromatic monooxyengases, esp. the Trp-5-monooxygenase...I've run a ClustalX protein sequence alignment on phenylalanine hydroxylase, Trp-5-monooxygenase and Tyr-4-monooxygenase and there is surprisingly high similarity between them all...one conserved region that you could recheck w/ a nucleotide sequence alignment is:
IGLASLGA which would come out as 24 bp in nucleotides...unfortunately I don't have any of these in nucleotide form at the moment so I can't calculate the degeneracy that would be present in the PCR primers, but the protein sequence is perfectly conserved here!
Whatcha think?


Begin with the dissolution of superfluous matters
So that desire and consciousness are free