POPTART
(Stranger) 08-07-03 07:33 No 452298 |
claviceps cultivation with tryptophan? | Bookmark | ||||||
In the Biochemical Data section there is a line that says "The addition of l-tryptophan in the cultures implies a remarkable increase of the quantity of the produced ergot alkaloids". Swim is guessing that this could also be a good idea for claviceps cultivation too. Any ideas? http://www.eleusis.ws/en/articles/vel-fu |
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Bubbleplate (Hive Bee) 08-07-03 12:32 No 452333 |
Some strains will | Bookmark | ||||||
supposedly, increase alkaloid production by adding tryptophan, and some won't, according to papers in various microbiological journals and Patents. You have to try it and test for alks. for each strain... |
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POPTART (Stranger) 08-08-03 08:57 No 452475 |
:-( So, we're looking at a thesis here. | Bookmark | ||||||
So, we're looking at a thesis here. This can become a laborious job to itentify especially if the strain being used is unknown. Thanks for your help |
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Bubbleplate (Hive Bee) 08-08-03 10:39 No 452484 |
Well, That's True For Growing Claviceps | Bookmark | ||||||
and getting alkaloids out of it. Like I've posted here before, it's easy to grow the fungus; harder to isolate and identify a strain that actually produces anything. The techniques used to identify strains that produce alkaloids are: 1) Color: Strains that produce purple or red coloration into the agar or liquid mediums are usually a sign of alkaloid production. 2) Chemical Testing - Test extract of fungus with para-dimethylaminobenzaldehyde (PDAB); i.e. Ehrlich-van Urk test. 3) Spectrometer - Test for alkaloids using a photospectrometer in the UV range |
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POPTART (Stranger) 08-11-03 05:20 No 452971 |
Thanks bubbleplate, SWIM, will stick to ... | Bookmark | ||||||
Thanks bubbleplate, SWIM, will stick to developing/finding a strain without tryptophan. From what SWIM has read, mutations can be induced with uv. That's one step of getting higher alkaloid producing strains. Thanks for your help |
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