mammIL (Stranger)
10-19-00 22:13
No 61538
      increasing tryptamine concentration in P. Cubes  Bookmark   

would adding tryptophan to the growth medium increase the tryptamine levels?  If so how much should be added?
 
 
 
 
    psyloxy
(Hive Bee)
10-20-00 02:14
No 61616
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

tryptamine and DMT are known to increase psiloc[yb]in content but I'm not sure if Trp aslo does it (though I'd really like to know!).

--psyloxy-- Yeah mdma is funny but has anyone of you ever experienced ayahuasca ?
 
 
 
 
    SPHYBRID
(Hive Bee)
10-20-00 17:52
No 61822
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

Ah, I believe that it would. Decarboxylation after the acetylation/methylation of the amine would be sufficient to allow the hydroxylation of the indole 4 position I believe maybe 5. One is bufotenine. Decarboxylaton is very spontaneous. Maybe find out the enzyme/covitamin that contributes to the decarboxylation. Lemme see I think it is the pyridoxine or phospahte of pyridoxine + basic buffer NH3OH There you go.....?

SPHYBRID
 
 
 
 
    SPHYBRID
(Hive Bee)
10-20-00 17:55
No 61823
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

Yeah psyloxy ahyuasca was a crazy time, already posted about it though. More of a LSA +m carbolines+lotso cannabis extract= very crazy indead but good if your into that type of thing.
 
 
 
 
    KrZ
(Hive Bee)
10-20-00 19:01
No 61838
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

Erowid, I believe, has the appropriate ratio of tryptophan to add per weight of substrate in order to approximately double psilocin output.  They also have a paper detailing addition of tryptamine to produce quadruple the amount of psilocin.

A note on tryptophan decarboxylation;
Use 454g tryptophan, 1150 mL tetralin, and aliquot 4 13 mL portions of acetone.  Using the longest path-length condensor available setup a refulx system with an addition funnel.  Place the tryptophan, a stribar, and the tetralin in a 2L RBF and add the acetone to the addition funnel.  Pump ice-cold water through the condensor, add the first portion of acetone and commence reflux.  After a timed period of 3-4 hours, add the second fraction of acetone through the addition funnel dropwise.  Assure that there is a continuous supply of ice condensor fluid availbe.  Repeat until all of the aliquots have been added and reflux for a total time of between 12 and 18 hours.
 
 
 
 
    Semtex
(Hive Elder/ Old Fart)
10-23-00 00:27
No 62484
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

Is there a post rxn workup/clean up proceedure, or just distill after the 12-18hours is up...?


crazycrazy
 
 
 
 
    foxy2
(Hive Addict)
10-23-00 09:02
No 62579
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

A simple acid base extraction should do the trick
 
 
 
 
    sparks
(Stranger)
10-30-00 02:49
No 64801
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

About that tryptophan thing, i think it was diskussed at the shroomery, and was found to make no increase in alkaloids,sorry.
 
 
 
 
    KrZ
(Hive Addict)
10-30-00 04:02
No 64825
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

I will believe published journal articles over your spun-out friends at the shroomery any day of the week.
 
 
 
 
    ProxYuk
(Stranger)
11-03-00 22:01
No 66389
      Re: increasing tryptamine concentration in P. Cubes  Bookmark   

I've heard of ayahuasca, isn't it a extract from a S. American bark? Is it legal and obtainable?


Dissociatives Opioids Psychedelics Entheogens
------DOPE-----=( ProxYuk )=-----DOPE------