Using phalaris with mushrooms (tryptamine cubensis , Hive Tryptamine Chemistry


		myco_chemist5 (Stranger) 10-03-03 12:20 No 462423				Using phalaris with mushrooms (tryptamine cubensis
						what if you grew Phalaris, extracted the DMT, and used it in some of the growth or casing on a batch of fungus. Would the DMT be converted to 4 OH DMT (psilocybin)? Thanks Grateful Growers, and their community service



		Rhodium (Chief Bee) 10-03-03 16:16 No 462460				Anabolic Tryptamines for Shrooms
						Yes, and even tryptamine would be converted to Psiloc(yb)in. UTFSE in this forum for details. Also note that phalaris grass contains a lot of 5-MeO-DMT beside its DMT content.



		moo (Hive Bee) 10-03-03 20:15 No 462478				Feeding mushrooms with precursors
						Biosynthesis of Psilocybin, Part II. Incorporation of Labelled Tryptamine Derivatives Stig Agurell, J. Lars G. Nilsson Acta Chemica Scandinavica 22, 1210-1218 (1968) Abstract: The biosynthesis of psilocybin has been investigated by feeding labelled precursors to Psilocybe Cubensis. The following specifially labelled compounds were synthesized: psilosin-³H, 4-hydroxytryptamine-¹⁴C, N,N-dimethyltryptamine-¹⁴C and -¹⁴C-³H, N-methyltryptamine-¹⁴C-³H and DL-tryptophan-³H. A number of other indoles were labelled by acid catalyzed exchange in tritiated water. The experimental data suggests a sequence: tryptophan --> tryptamine --> N-methyltryptamine --> N,N-dimethyltryptamine --> psilocin --> psilocybin. The fungus can also by an alternative route convert 4-hydroxytryptamine to psilocybin. Large differences in the rate of absorption of different closely related precursors has been observed. A relevant quote from the article: However, the results in Table 1 show that 4-hydroxytryptophan which so far has not been encountered in nature, in contrast to tryptophan does not function as a precursor of psilocybin. Tryptamine, which is readily formed from tryptophan by P. Cubensis, serves as a better precursor of psilocybin than tryptophan. If tryptamine is methylated to N-methyltryptamine, this is an even better progenitor of psilocybin with incorporations showing that more than half of the psilocybin was derived from the introduced labelled precursors (Expts. Nos. 38, 42). The corresponding dimethyl derivative N,N-dimethyltryptamine may, however, be considered as a poor precursor as judged from the incorporation percentages. The culture conditions they used was a submerged culture, described in Acta Pharm. Suecica 3, 67 (1966). To find out why DMT was incorporated so poorly although it is a close relative to psilocin, they measured the amounts of uptake of precursors from medium by P. Cubensis. The uptakes are ordered as follows: psilocin (ca. 80%) > tryptamine (ca.65%) > 4-hydroxytryptamine (ca. 40%) > N-methyltryptamine (ca. 30%) > DL-4-hydroxytryptophan (ca. 25%) > DL-tryptophan (ca. 20%) > N,N-dimethyltryptamine (ca. 5%). The values are measurements after 26 hours, the time after which most of the uptake had already happened. If the DMT doesn't get absorbed, it is no wonder it's not incorporated into psiloc(yb)in. Scanning the whole article is also possible. Therefore, it might be fruitful to find out plants that contain N-methyltryptamine or/and tryptamine. _{fear fear hate hate}



		Rhodium (Chief Bee) 10-04-03 09:30 No 462566				Acta Chemica Scandinavica 22, 1210 (1968)
						Here is the full article quoted from above: Post 453575 (Rhodium: "Ref 12: Biosynthesis of Psilocybin", Tryptamine Chemistry)



		moo (Hive Bee) 10-04-03 10:12 No 462573				Great! :-)
						Great! _{fear fear hate hate}