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timnickbear
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Joined: 21 Jun 2005 |
Posts: 30 |
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990.20 Points
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re: Claviceps purpurea yield confusion cleared (high bandwid
Mon Jul 11, 2005 11:57 pm |
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guess I am going to have to get the old biotech of filamentous fungi book out for all of you to see how to correctly do the culture as of recent, not saying this liturature isn't informative. The process is better done in front of a laminar pref to avoid contams, and usually when stock culture isn't used gentamycin sulfate is used in agar to kill off bacteria allowing for a sterile culture to be obtained. A t-s slant is pref to get it to grow before liquid culture is innocculated by the fungus. |
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re: Claviceps purpurea yield confusion cleared (high bandwid
Tue Jul 12, 2005 1:32 am |
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That would be awesome, please do. Before all this, it seemed like some people randomly said claviceps worked great and some randomly said it didn't. |
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timnickbear
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Joined: 21 Jun 2005 |
Posts: 30 |
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990.20 Points
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Alice
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Joined: 10 Feb 2005 |
Posts: 27 |
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783.38 Points
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timnickbear
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Joined: 21 Jun 2005 |
Posts: 30 |
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990.20 Points
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re: Claviceps purpurea yield confusion cleared (high bandwid
Wed Jul 13, 2005 2:17 am |
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Can some one please find:
Mantle, P. G. (1975) in The Filamentous Fungi: Vol. 1, Industrial Mycology (Smith, J. E., and Berry D. R., eds.), pp. 281-300, John Wiley, New York. |
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timnickbear
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Joined: 21 Jun 2005 |
Posts: 30 |
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990.20 Points
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re: Claviceps purpurea yield confusion cleared (high bandwid
Wed Jul 13, 2005 2:22 am |
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Finkelstein, D.B. & Ball, C., eds. (1992). Biotechnology of Filamentous Fungi: Technology and Products. Butterworth-Heinemann, Boston. pp. 265-289 |
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timnickbear
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Joined: 21 Jun 2005 |
Posts: 30 |
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990.20 Points
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re: Claviceps purpurea yield confusion cleared (high bandwid
Wed Jul 13, 2005 2:22 am |
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Those should get you off into a good read ![Smile](images/smiles/icon_smile.gif) |
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Alice
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Joined: 10 Feb 2005 |
Posts: 27 |
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783.38 Points
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re: Claviceps purpurea yield confusion cleared (high bandwid
Wed Jul 13, 2005 4:43 am |
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well dono if thats exactly what u looking for but its good one and more specific one than Biotechnology of Filamentous Fungi
here u go
Biotechnology_and_genetics_of_ergot_alkaloids |
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timnickbear
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Joined: 21 Jun 2005 |
Posts: 30 |
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990.20 Points
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re: Claviceps purpurea yield confusion cleared (high bandwid
Thu Jul 14, 2005 2:23 am |
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oh wow thats a good read. The one by mantle describes how to get 5mg/ml of erogtamine in a submerged culture. More of the actual nutrient workup. You had a good read though. Can we get that in the archives? |
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brain
Linguist Extraordinaire
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Joined: 08 Mar 2005 |
Posts: 143 |
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2405.16 Points
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re: Claviceps purpurea yield confusion cleared (high bandwid
Sun Jul 17, 2005 12:48 am |
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Can enyone have more pages form book from 1-st post ??? mabey 190...x pages ? there is writen how to select claviceps culture... mabey someone know how to do it? to indicate and select culture that giwes highest yelds of ergotamine? |
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