|
Author |
Message |
|
Hairy root disease
Tue Feb 15, 2005 9:10 pm |
|
|
Look here:
http://www.esf.edu/efb/course/EFB530/lectures/agrobact.htm
Quote: |
causes proliferation of tissue, like cancer growth...
|
The infection of certain plants with agrobacteria to produce edible roots en masse is used in Japan on a commercial scale for a long time.
The roots are grown in tanks of wanter with nutrients, the roots form big floating balls in very short time. This way roots of plants are produced which take a long time to grow in nature.
We all know a tree which has a interesting essential oil in its roots, in special in the bark of the roots - the Sassanfras tree.
Now two questions arise:
- Is it possible to infect Sassanfras with agrobacteria?
- Will the infected root still contain considerable amounts of safrole?
If both questions can be answered with yes, this would be a comparable simple way to grow Sassanfras roots with a lot of bark in some weeks in a well temperated bucket with water and nutrient salts. Whats not bad compared with the years and the space a tree needs to grow.
I dont know more than posted here.
So please dont post QUESTIONS - search and post further information.
regards
/ORG |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
|
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
jimwig
|
Joined: 12 Feb 2005 |
Posts: 2 |
|
0.00 Points
|
|
Wed Feb 16, 2005 8:34 pm |
|
|
man this is very exciting stuff. imagine a greenhouse growing sassafrass roots by the megagram!!!!
i am an idiot BUT an open minded one.
dr organikkum - please share more with us. I for one could launch in a microexperimental mode within moments. (simile)
very very promising.
jimwig
edit --"Agrobacterium-mediated transformation is only effective among plants that the bacterium can infect
* this includes most dicots"--
seems our tree is indeed a dicot!!! |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
|
Thu Feb 17, 2005 3:22 am |
|
|
This is very interesting. Apparently, bacterial DNA becomes integrated into the plant genome via a plasmid and causes a cancer-like condition in the plant. The same thing happens with crown-gall disease. |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
|
Thu Feb 17, 2005 6:55 am |
|
|
This seems to be the strain being used (although there are many varieties):
Rhizobium rhizogenes
http://www.dsmz.de/species/sp200090.htm
I suspect you would need some kind of hydroponic style system with bubbler, temperature control and balanced nutrients to grow the roots. |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
stooge
|
Joined: 11 Feb 2005 |
Posts: 56 |
|
1401.36 Points
|
|
Mon Feb 21, 2005 2:49 pm |
|
|
hey - don't want to bother, but - this might also work with poppy roots, i guess?!?
**edit:*******
oh well, of course it's again nearly impossible to obtain these things without being an official lab-whatever ...
i hate them ![Evil or Very Mad](images/smiles/icon_evil.gif) |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
stooge
|
Joined: 11 Feb 2005 |
Posts: 56 |
|
1401.36 Points
|
|
Wed Feb 23, 2005 1:50 pm |
|
|
what i wonder is, if it might be possible to grow plants in water with nutrients alone without drowning them - even if it takes much longer it might still be worth the while in my eyes. it seems pretty useless to me, but if it works with agrobacteria i think i'll give it a try, even if i feel a little dull with that...
in the end it's nothing else than hydroponics only without the "stones" ...
hm, no clue. will report if it works anyhow. |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
stooge
|
Joined: 11 Feb 2005 |
Posts: 56 |
|
1401.36 Points
|
|
Sat Mar 12, 2005 3:48 am |
|
|
Quote: |
The infection of certain plants with agrobacteria to produce edible roots en masse is used in Japan on a commercial scale for a long time.
The roots are grown in tanks of wanter with nutrients, the roots form big floating balls in very short time. This way roots of plants are produced which take a long time to grow in nature.
|
@organikum or anyone: does anyone know the appropriate technical term for the method mentioned in the first post?
Trying to find out more on that via google, and so on ...
BTW: Maybe one could try some genetical experiments with agrobacterium tumefaciens, maybe transfering genes between interesting plants and inconspicious ones? (nudge, nudge) |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
loki
guinea pig
|
Joined: 09 Mar 2005 |
Posts: 391 |
|
14167.88 Points
|
|
Wed Mar 16, 2005 11:28 am |
|
|
forgot to mention mimosa hostilis and acacia obtusifolia here, as well as desmanthus illinoensis here...
It can't be that hard to get a hold of this organism to promote this disease in roots of plants, surely (?)
syrian rue produces useful amounts of alkaloids in its roots too...
perhaps there is other means to promote this kind of cancer-like illness in a plant than bacteria, seems like a recipe for disaster for the average plant enthusiast to infect one plant with it, before you know it everything will be infected.
edit: i read somewhere that an excess of boron in the nutrients fed to a plant can promote excessive bulking out of plants (just wanted to add this as it may be relevant) |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
cascabel
|
Joined: 06 Apr 2005 |
Posts: 4 |
Location: not for you to know. |
129.86 Points
|
|
Fri Apr 08, 2005 1:48 pm |
|
|
Hello!
Agrobacterium tumefaciens, a.k.a the crown gall bacillus, in itself, will not yield the edible roots. The Japanese team, if I understand their procedure correctly, used a genetically-modified strain of Agrobacterium for their experiment. I do not think, however, that genetic modification is in the grasp of the home chemist as of yet.
MargaretThatcher explained in an earlier post how the crown-gall bacillus performs genetic modification on the plant, so I will not elaborate further. It is my understanding that what made the infected roots edible was whatever was inserted into the bacterial plasmids.
QUOTE:
"...roots are grown in tanks of water with nutrients..."
That would be the subject of hydroponics.
the cascabel (^_^) |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
|
Fri Apr 08, 2005 4:39 pm |
|
|
Rhizobium/agrobacterium are different names for the same species. The bacterium is naturally occuring in the soil and enters the root through injuries to cause hairy root disease. I imagine it would be very difficult to isolate and culture without expert knowledge. Commercial strains are available. |
|
Back to top |
|
![](templates/subSilver/images/spacer.gif) |
|
|